BTEC 5338 Methods in Biotechnology
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Let's have a brief discussion about the course:
The BTEC 5338 Methods in Biotechnology course is conventionally divided into two parts: gene cloning and expression and characterization of recombinant proteins. Each section contains two modules. Gene cloning modules I and II include cloning and sub cloning of foreign genes. Modules III, IV and V cover over expression, purification and characterization of recombinant proteins, respectively. Beneath this course, you will get reading assignments, supplementary materials, required experiment logs, and course announcements as needed.
Well-prepared students will always be able to use various bioinformatics tools to find genes of interest; be able to design structures that can be used to over express proteins; be able to isolate and purify these plasmids and perform restriction digest analysis on the purified plasmids; be able to transform bacterial cells and use antibiotic resistance to screen for transformed cells; will be able to isolate DNA and analyze sequencing data; will be able to release expressed proteins from bacterial cells and purify proteins using various chromatographic methods; will be able to use gel electrophoresis to control the purity of DNA and proteins and assess their molecular weight; will be able to measure DNA and Protein concentration and measure enzyme activity to calculate specific activity.
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